Mindless vandals have wrecked three buses in less than a fortnight.
The £184,000 luxury Stagecoach Gold buses started running on service 10 from December 8.
But just days later the gold double deckers were attacked.
If the vandalism doesn't stop, Stagecoach is threatening to remove them from the service.
The buses were so badly vandalised that they have had to be withdrawn from service.
Staff and police are poring over CCTV footage in a bid to catch the vandals.
A spokesperson for Stagecoach West said: "We are all absolutely devastated by the behaviour of a few mindless idiots which unfortunately could impact on the vast majority of well behaved customers.
"If this vandalism does not cease immediately then we as a company will be left with no choice than to take these luxury vehicles off the road.
"This type of problem has not happened anywhere else where the Gold brand has been introduced and is not going to be allowed to happen in Gloucester.
"Stagecoach Gold is a reward for our loyal customers who continue to use our services on a regular basis.
"It is therefore such a shame that the actions of a few could have such a negative effect on so many people."