Last orders have already been called at the much-loved Ridge and Furrow pub in Abbeydale, which closed for the final time on Sunday.
The pub in Glevum Way was due to close earlier this year to make way for a Morrisons filling station.
Delays in the planning application meant it was set to stay open until the new year.
However landlord Steve Luckett said it was not making enough profit to stay open.
Steve had been running the Ridge and Furrow on a weekly contract since March.
That meant that staff would have had to vacate the premises within a week of notice being given.
Steve, who has been running the pub for 13 years, said: “It was an emotional day and tears were shed but it was with great regret that I had to close. “The overheads were too much for me.
“We put on regular bands but the last one cost me £600. With security, I was not making enough profit.
“This year we have only had 20 bookings for Christmas, where as in the past we have had 1,000. Some nights it is really busy but then other nights it is really quiet.
“I have to pay £500 in business rates and a lot for electricity. Insurance has also gone up to £6,000.”
Steve was paying reduced rent to Trust Inns for the past few months.
He added: “In its heyday we employed around 20 to 30 staff but now we only had eight left.”
Employees at the Ridge and Furrow were given the choice of transferring to the Beacon Inn at Haresfield, which Steve and his family started running in May this year.
Steve added: “I went to the Ridge and Furrow on Sunday to let staff know it was closing, we enjoyed a Chinese meal and this week some staff are due to sign a contract at the Beacon Inn.”
Pub regular Jason Cooper said: “The sudden closure does not surprise me one little bit, it sums up the last two years. For all the good times and memories I have had in the Ridge, it has killed me having to watch it go downhill from no investment or facelift for years, to Morrisons wanting to build a patrol station on the site.”
Ben Brown, another pub goer, said: “It has been my local for nine years. It was probably the best non-city centre pub in Gloucester. What a waste.”
In August, 2,000 residents signed the petition to save the pub from closure.
In October resident Andrea Hawkins submitted a bid to turn the it into a community asset but was rejected.
Former employee Nik Hill said: “I worked there from 1982 until 1986. In May 1983, it burnt down because of problems with gas works. It reopened in October but the atmosphere was never the same. During the opening year it was heaving.”