Unions are threatening strike action as 600,000 NHS workers will be losing out on a pay rise.
The government announced a basic one per cent wage increase for members of the armed forces, prison officers and judges.
But health service unions are considering industrial action as thousands of nurses and other staff will not be getting the further rise.
The Department of Health announcement means those who automatically receive progression-in-job increases to their pay will not get the salary boost.
It is unclear exactly how many workers in Gloucestershire will be affected by the move.
But it is believed more than 1,500 nurses and around 2,500 other workers, running Gloucestershire Royal and Cheltenham General hospital, could miss out on the extra cash.
Around 7,500 people are employed at the county’s two main hospitals, including 2,182 nurses.
It is estimated that around 70 per cent of nurses and 55 per cent of other workers will be missing out on the pay hike.
The annual progression-in-job increment, which amounts to around three per cent, is given as workers gain more skills.
One of the county’s nurses, who did not wish to be named, said: “It seems crazy that we are told we are getting more money because we are learning and developing new skills.
“But then they use that against us and as a reason not to give us this basic salary rise.”
The Royal College of Nursing has been angered by the decision, particularly as it has ignored a recommendation from the NHS Pay Review Body.
Dr Peter Carter, chief executive of the RCN, said: “To suggest that incremental pay is the same as a pay uplift, or that NHS staff are simply being rewarded for time served is to either deliberately mislead or to fundamentally misunderstand how NHS pay works.
“Incremental pay is used in the private and public sectors and is only awarded to NHS staff when they can demonstrate that they have developed their skills.”
Union Unite is consulting its members on possible industrial action as it believes that around 55 per cent of the NHS workforce will lose out from April 1.
Unite head of health Rachael Maskell, said: “Jeremy Hunt (the Heath Secretary) has adopted a divide and rule tactic.
“He is deliberately muddying the waters by trying to imply that the annual increments that staff receive, as they gain more skills to benefit patients throughout their careers, is part of the annual pay increase – it is not.
“It is despicable that Hunt has adopted such an underhand tactic.
“Our members are being hit by the escalating cost of living crisis – their pay packets are shrinking year by year.
Rehana Azam, GMB national officer, added: “GMB members will be outraged with Government isolating NHS workers like this.
“They are attacking not only living standards but also the agreed way pay is set in the NHS. This makes a ballot for industrial action all the more likely.”
Unison’s head of health, Christina McAnea, said: “It’s a disgrace that 70 per cent of nurses and midwives will not even get a pay rise this year – what sort of message does this give to the value this Government places on dedicated NHS staff?”
But David Cameron defended the decision. The Prime Minister said: “It is right to make difficult decisions about public sector pay. It is good that it is increasing and not frozen.
“But it is right to take these difficult decisions because it means that we can keep more people employed, more people in work, we can make sure we spend money on vital treatments, on hospitals, on delivering services which is what patients so badly want.”