Car sales at Naas Lane in Quedgeley are to be investigated by Gloucester City Council.
Parish councillors say they are worried about the roadside activity.
Councillor Anna Mozul, chairman of Quedgeley Parish Council, said: "I have seen cars constantly being sold on there."
Gloucester city councillor Andrew Lewis said: "We will get the enforcement officers out to see what can be done about it.
"It is always quite difficult because as soon as you remove them, a week later they are back again."