GLOUCESTER's tenacity paid off - the Cherry and Whites secured a 22-19 victory over London Irish at the Madejski Stadium.
Irish out-scored Gloucester three tries to one, but it was the boot of Billy Twelvetrees that secured victory for the visitors.
Twelvetrees kicked four penalties and successfully converted winger Jonny May's try, before under-fire Fredde Burns came on deep into the second half and took over kicking duties.
Though he missed a drop goal attempt in the final minute, he successfully kicked one penalty over to add to the points haul.
It was a solid performance from the Cherry and Whites despite a spirited comeback from Irish in the second half thanks to two tries from centre Eamonn Sheridan, but Gloucester ensured the spoils went home to Kingsholm.
Gloucester led London Irish 16-7 at halftime, after a Jonny May try in the 28th minute and three penalties from the boot of Billy Twelvetrees.
Irish scored a try after just 63 seconds, as Fergus Mulchrone went over, with Ian Humprehys adding the extras.
Sheridan scored a try in the 60th minute, but Humphreys conversion sailed wide, before the centre scored again in the 73rd minute with Shane Geraghty adding the extras.
But the replacement kicker got a penalty attempt wrong with just three minutes left on the clock, and Irish's hopes of at least a draw were gone.
A 12-6 derby victory at Kingsholm over Worcester last weekend elevated Gloucester to ninth in the league,and they remain in this position after today's victory. Meanwhile Irish have slipped to 11th after just two wins this season.
Director of Rugby Nigel Davies kept his faith in the XV who defeated Worcester, but hooker Darren Dawidiuk has since had to pull out with injury (knee), with Huia Edmonds replacing him in the front row.
Rob Cook; Charlie Sharples, Henry Trinder, Mike Tindall, Jonny May; Billy Twelvetrees (capt), Jimmy Cowan; Nick Wood, Huia Edmonds, Sila Puafisi; Elliott Stooke, James Hudson; Matt Cox, Matt Kvesic, Ben Morgan
Koree Britton, Yann Thomas, Shaun Knight, Will James, Gareth Evans, Dan Robson, Freddie Burns, Martyn Thomas
James O'Connor, Topsy Ojo, Fergus Mulchrone, Eamonn Sheridan, Alex Lewington, Ian Humphreys, Tomás O'Leary, Matt Parr, Jimmy Stevens, CJ Van Der Linde, George Skivington (c), Ian Gough, Kieran Low, Ofisa Treviranus, Blair Cowan
Replacements: Mike Mayhew, John Yapp, Leo Halavatau, Nic Rouse, Jebb Sinclair, Andrew Fenby, Shane Geraghty, Ed Hoadley