TRADERS say their nightmare is over as Kingsholm Road reopened following months of roadworks.
Businesses along the road have estimated their losses at tens of thousands of pounds since June, when Severn Trent moved in to carry out vital sewer and flood alleviation work.
Motorists were allowed through again on Saturday for the first time since the work began.
Geoff Cooper, owner of the Cooper Cowen car dealership in the street, said: "It's fantastic news, it means that we can now start business again and try and claw back what we lost in the last four months.
"We've been standing still, we've lost a large amount."
Stan Gardner, of John Stayte Services, said: "It's really welcome to see it open again.
"We've been getting so many phone calls in the past few months because people just didn't think we were open and it was extremely difficult to actually get to us, it's been terrible."
Businesses have been told they can apply for compensation from Severn Trent.
Benita McLean, managing director of The Garage, Gloucester, said: "It's been horrendous, but we are just hoping for support from the houses nearby to get back to it."
Adrian and Noelle Johnson also saw a serious slump in trade at their Mantra Motorcycles business.
"It's joyous that the road is open again, we're over the moon," said Noelle.
"It's been really bad, the message has been to avoid Kingsholm for the last four months so we just want the message out there that we are here."
Severn Trent will return in the new year to finish off minor bits and pieces, but the road will stay open.
A spokesman said: "Our work to replace the sewer on Kingsholm Road is now complete.
"This has been a large part of our £14.5million project to replace sewers in Gloucester, which will see homes in the area being taken off the flood risk register.
"We would like to thank the local community for their patience while we completed this work, particularly the local businesses, who may have seen an impact on their trade.
"We are now working with the local businesses to compensate them for any loss of profits they may have suffered as a direct result of our work."