Passionate opponents of the badger cull met in Coleford last night.
More than 100 people gathered at the Bells Hotel to hear animals rights leaders talk speak against the plans.
Queen legend Brian May had been due to attend the meeting but had to pull out at the last minute because of a family tragedy.
Chief executive of the RSPCA Gavin Grant spoke and said: "This is being done in our communities and to our wildlife but is it being done in our name?"
Many of the audience shouted "no".
Mr Grant added: "If they cull the badgers we will never forget and never forgive."
The Government says culling badgers will help combat bovine tuberculosis, which costs the UK more than £100m per year.
It plans to issue licences to land owners in Gloucestershire and Somerset so they can shoot badgers with high velocity rifles at night as part of a pilot cull.
Campaigners say vaccination and better management of cattle is the solution.
Earlier this week the Badger Trust lost its case in the Court of Appeal to have the cull proposal thrown out.
A statement from Mr May was read out about what he described as the "tragically misjudged" cull.
In it he urged people to spread the word about a petition at www.TeamBadger.org
If it gets 100,000 signatures the issue will be eligible for debate in the House of Commons.
Mr May said: "We may lose some battles, but, by God, we will not lose the war."