DNA analysis of suspected big cat fur in the county reveals it's from a domestic moggie.
University of Warwick,conducted a further test on hair taken from an empty building where a suspected big cat was sighted near Dursley.
This is after its experts found evidence only of foxes on two deer carcasses found near Dursley and Woodchester.
Dr Robin Allaby of the School of Life Sciences said yesterday: "Our analysis of a sample of a pinch of fur taken from a site near Dursley shows that it had the genotype of a domestic cat."
Meanwhile a skeleton believed to be of a big cat found in Cornwall has been identified as a badger by Gloucester big cat tracker Frank Tunbridge.