Police officers closed off the part of the A417 near the Air Balloon Roundabout after a three-vehicle crash on Sunday evening.
Emergency services rushed to the A417 near the roundabout, towards Nettleton Bottom, after reports of a crash around 5.20pm.
Three cars, a grey BMW three series, a white Nissan Qashqai, and a blue Vauxhall Astra were involved in the smash near the infamous bottleneck.
A spokesman for the police said the drivers and passengers were not seriously injured, although paramedics were at the scene.
The road at the Air Balloon Roundabout towards Nettleton Bottom was closed in both directions as the cars are being brought to the side of the road while waiting for recovery.
Businesses, political parties and various organisations have come together to try to find a solution to the long-term congestion problems at the roundabout.
A statement released by Gloucestershire Fire and Rescue Service said one man and one woman were released before the arrival of fire crews. One further adult female was released by the fire service.
Three people were taken to hospital by South West Ambulance Service.